Refining the genetic architecture of flag leaf glaucousness in wheat.

KEY MESSAGE: The cuticle is the plant's barrier against abiotic and biotic stresses, and the deposition of epicuticular wax crystals results in the scattering of light, an effect termed glaucousness. Here, we dissect the genetic architecture of flag leaf glaucousness in wheat toward a future targeted design of the cuticle. The cuticle serves as a barrier that protects plants against abiotic and biotic stresses. Differences in cuticle composition can be detected by the scattering of light on epicuticular wax crystals, which causes a phenotype termed glaucousness. In this study, we dissected the genetic architecture of flag leaf glaucousness in a panel of 1106 wheat cultivars of global origin. We observed a large genotypic variation, but the geographic pattern suggests that other wax layer characteristics besides glaucousness may be important in conferring tolerance to abiotic stresses such as heat and drought. Genome-wide association mapping identified two major quantitative trait loci (QTL) on chromosomes 3A and 2B. The latter corresponds to the W1 locus, but further characterization revealed that it is likely to contain additional QTL. The same holds true for the major QTL on 3A, which was also found to show an epistatic interaction with another locus located a few centiMorgan distal to it. Genome-wide prediction and the identification of a few additional putative QTL revealed that small-effect QTL also contribute to the trait. Collectively, our results illustrate the complexity of the genetic control of flag leaf glaucousness, with additive effects and epistasis, and lay the foundation for the cloning of the underlying genes toward a more targeted design of the cuticle by plant breeding.

A comparative study on the artificial UV and natural ageing of beeswax and Chinese wax and influence of wax finishing on the ageing of Chinese Ash (Fraxinus mandshurica) wood surfaces.

This study investigated and compared the behaviour of two types of natural waxes, beeswax and Chinese wax, by means of two different ageing tests: an artificial accelerated ageing test using UV light and a simulated natural ageing test (indoors conditions) based on the action of natural, window-filtered light. The same tests were employed to evaluate the influence of wax finishing on the ageing behaviour of Chinese Ash (Fraxinus mandshurica) wood surfaces. Ageing effects were evaluated by direct and microscopic observation, colour measurements in the CIELab system and FTIR investigations. The results yielded by both accelerated UV ageing (72 h) and simulated indoors natural ageing (6 months) indicate that the waxes under analysis here are materials with a good ageing resistance. FTIR investigation revealed only minor chemical changes following ageing, more evident with beeswax than with Chinese wax. Finishing Chinese Ash wood surfaces with the two types of waxes influenced their ageing behaviour in terms of colour and surface chemistry changes. For both UV and natural ageing the maximum colour differences occurred for the samples finished with Chinese wax. The colour differences ΔE after 72 h accelerated UV ageing were in all the cases higher than those occurring after 6 months of indoors simulated natural ageing. Acceleration indexes for UV exposure compared to exposure to natural window-filtered sunlight, of about 40X-60X, were calculated. Both UV accelerated ageing and natural simulated ageing resulted in significant surface chemistry changes for the unfinished Chinese ash wood samples, consisting mostly in lignin degradation and formation of carbonyl containing chromophores. FTIR investigation of wax finish wood samples revealed only very minor chemical changes of the top wax layers, although chemical changes occurring beneath the coating layer on the wood surface are highly probable. Overall, the experiments presented in this paper indicate that Chinese wax seems slightly more resistant to ageing than beeswax.

Characterization of Ivoclar Vivadent Dental Restoration Material for 137CS Retrospective Radiation Dosimetry.

Retrospective dosimetry is the method of using materials on or near a person who is exposed to ionizing radiation to determine the amount of radiation received by the person. A possible candidate material for retrospective dosimetry is Ivoclar Vivadent IPS e.max® CAD ceramic dental restoration material, which exhibits radiation-induced thermoluminescence when exposed to gamma- and x-ray radiation from a Cs source. The purpose of this paper is to characterize the material and study the behavior of the thermoluminescence signal with radiation dose and with delay time between radiation exposure and thermoluminescence measurement. The first glow peak is well-modeled by a first-order glow curve deconvolution formula. The height of the first glow peak is approximately linear with dose. The fading of the signal with time is approximately described by a power law curve with cutoff. The material appears to be suitable for retrospective radiation dosimetry.

Accelerated dissipation of the herbicide cycloxydim on wax films in the presence of the fungicide chlorothalonil and under the action of solar light.

Photolysis is a known dissipation pathway of pesticides on leaves just after their spraying. This pathway may be affected by the residues of other pesticides. To illustrate this idea, this study investigated the mutual effect of two pesticides (chlorothalonil and cycloxydim) under simulated solar light. Cycloxydim was added at the agricultural rate (200 g ha(-1)) and chlorothalonil at 1.3-10% of the rate (20-150 g ha(-1)). These compounds were studied either pure or in their commercial formulation. Both analytical and kinetic data show that chlorothalonil significantly accelerates the decay of cycloxydim on wax films, promoting its oxidation, even at the lowest tested dose. Conversely, cycloxydim does not affect the fate of chlorothalonil. Moreover, the detection of oxidized forms of wax alkanes in the extracts demonstrates that chlorothalonil may have also a degrading effect on the leaves' constituents under the action of solar light.

Is wax equivalent to tissue in electron conformal therapy planning? A Monte Carlo study of material approximation introduced dose difference.

With CT-based Monte Carlo (MC) dose calculations, material composition is often assigned based on the standard Hounsfield unit ranges. This is known as the density threshold method. In bolus electron conformal therapy (BolusECT), the bolus material, machineable wax, would be assigned as soft tissue and the electron density is assumed equivalent to soft tissue based on its Hounsfield unit. This study investigates the dose errors introduced by this material assignment. BEAMnrc was used to simulate electron beams from a Trilogy accelerator. SPRRZnrc was used to calculate stopping power ratios (SPR) of tissue to wax, SPR (tissue) (wax), and tissue to water, SPR(tissue) (water), for 6, 9, 12, 15, and 18 MeV electron beams, of which 12 and 15MeV beams are the most commonly used energies in BolusECT. DOSXYZnrc was applied in dose distribution calculations in a tissue phantom with either flat wax slabs of various thicknesses or a wedge-shaped bolus on top. Dose distribution for two clinical cases, a chest wall and a head and neck, were compared with the bolus material treated as wax or tissue. The SPR(tissue) (wax) values for 12 and 15MeV beams are between 0.935 and 0.945, while the SPR(tissue) (water) values are between 0.990 and 0.991. For a 12 MeV beam, the dose in tissue immediately under the bolus is overestimated by 2.5% for a 3 cm bolus thickness if the wax bolus is treated as tissue. For 15 MeV beams, the error is 1.4%. However, in both clinical cases the differences in the PTV DVH is negligible. Due to stopping power differences, dose differences of up to 2.5% are observed in MC simulations if the bolus material is misassigned as tissue in BolusECT dose calculations. However, for boluses thinner than 2 cm that are more likely encountered in practice, the error is within clinical tolerance.

The effects of stress on plant cuticular waxes.

Plants are subject to a wide range of abiotic stresses, and their cuticular wax layer provides a protective barrier, which consists predominantly of long-chain hydrocarbon compounds, including alkanes, primary alcohols, aldehydes, secondary alcohols, ketones, esters and other derived compounds. This article discusses current knowledge relating to the effects of stress on cuticular waxes and the ways in which the wax provides protection against the deleterious effects of light, temperature, osmotic stress, physical damage, altitude and pollution. Topics covered here include biosynthesis, morphology, composition and function of cuticular waxes in relation to the effects of stress, and some recent findings concerning the effects of stress on regulation of wax biosynthesis are described.

Radiological properties of a wax-gypsum compensator material.

In this paper the radiological properties of a compensator material consisting of wax and gypsum is presented. Effective attenuation coefficients (EACs) have been determined from transmission measurements with an ion chamber in a Perspex phantom. Measurements were made at 80 and 100 cm source-to-skin distance (SSD) for beam energies of 6, 8, and 15 MV, for field sizes ranging from narrow beam geometries up to 40 x 40 cm2, and at measurement depths of maximum dose build-up, 5 and 10 cm. A parametrization equation could be constructed to predict the EAC values within 4% uncertainty as a function of field size and depth of measurement. The EAC dependence on off-axis position was also quantified at each beam energy and SSD. It was found that the compensator material reduced the required thickness for compensation by 26% at 8 MV when compared to pure paraffin wax for a 10 x 10 cm2 field. Relative surface ionization (RSI) measurements have been made to quantify the effect of scattered electrons from the wax-gypsum compensator. Results indicated that for 80 cm SSD the RSI would exceed 50% for fields larger than 15 x 15 cm2. At 100 cm SSD the RSI values were below 50% for all field sizes used.

Effects of ultraviolet-B radiation on cotton (Gossypium hirsutum L.) morphology and anatomy.

Cotton (Gossypium hirsutum L.) crop, cultivated between 40 degrees N and 40 degrees S, is currently experiencing 2-11 kJ m-2 d-1 of UV-B radiation. This is predicted to increase in the near future. An experiment was conducted to study the effect of enhanced UV-B radiation on vegetative and reproductive morphology and leaf anatomy of cotton in sunlit, controlled environment chambers. From emergence to harvest, cotton plants were exposed to 0, 8 or 16 kJ m-2 d-1 of UV-B in a square wave approach for 8 h from 0800 to 1600 h. Changes in plant height, internode and branch length, mainstem node number, leaf area, length and area of petals and bracts, and anther number per flower were recorded. Epidermal cell and stomatal density, stomatal index, leaf thickness, and epidermal, palisade and mesophyll tissue thickness were also measured. Initial chlorotic symptoms on leaves turned into necrotic patches on continued exposure to enhanced UV-B. Exposure to high UV-B reduced both vegetative and reproductive parameters and resulted in a smaller canopy indicating sensitivity of cotton to UV-B radiation. Enhanced UV-B radiation increased epicuticular wax content on adaxial leaf surfaces, and stomatal index on both adaxial and abaxial leaf surfaces. Leaf thickness was reduced following exposure to UV-B owing to a decrease in thickness of both the palisade and mesophyll tissue, while the epidermal thickness remained unchanged. The vegetative parameters studied were affected only by high levels of UV-B (16 kJ m-2 d-1), whereas the reproductive parameters were reduced at both ambient (8 kJ m-2 d-1) and high UV-B levels. The study shows that cotton plants are sensitive to UV-B at both the whole plant and anatomical level.

Determination of the relative linear collision stopping power and linear scattering power of electron bolus material.

The linear collision stopping power and linear scattering power for machineable wax relative to water have been determined for electron energies between 2 and 20 MeV. Knowledge of these quantities is necessary for the use of this wax as bolus in electron pencil-beam dose algorithms. The atomic composition of the wax (rho = 0.920 +/- 0.001 g cm(-3)) was obtained by having the wax assayed. The formalisms expressed in the ICRU Report 35 were used to calculate the relative linear collision stopping and linear scattering powers of the wax. The calculated relative linear collision stopping powers of 2 to 20 MeV electrons in the wax ranged from 0.949 +/- 0.005 to 0.952 +/- 0.005, and the calculated relative linear scattering powers ranged from 0.734 +/- 0.004 to 0.729 +/- 0.004. As a check of the calculation method, the relative linear collision stopping power was measured by determining the shift in electron central-axis depth-ionization curves when varying thicknesses of water were replaced by wax. These measurements, made using 10, 12, 15 and 18 MeV electron beams with wax thicknesses from 1.0 - 4.0 cm, resulted in a mean value of 0.931 +/- 0.008. Determination of the relative linear stopping power and the linear scattering power by using the measured CT number to extract values from patient data tables resulted in values of 0.933 +/- 0.009 and 0.746 +/- 0.016, respectively, indicating that it should be acceptable to use the Hounsfield values obtained with CT scans for treatment planning dose calculations.